beautypg.com

3 individual blanking, Individual blanking – Eppendorf G0.5 µPlate User Manual

Page 11

background image

Operation

Eppendorf

®

μPlate G0.5

English (EN)

11

5.2.3

Individual Blanking

All wells which will be used for the subsequent measurements will be included in the
determination of individual blank values (individual blanking). For each well used, the
information of the corresponding blank value is saved. The blank value correction of the
samples is carried out using the corresponding individual blank value (of the affected
well) instead of an average blank value.

Abb. 5-2:Individual Blanking checkbox

Fig. 5-2:

Individual Blanking checkbox

We recommend using "Individual Blanking" as the default option for blanking because
this mode delivers the most precise and reliable results.

The blanking information is retained in the software until:

• The PlateReader is disconnected from the computer

• The software is exited.

• A different sample type is selected

If other sample types are selected, the blanking procedure must be repeated with the
corresponding wavelengths.

(see Fig. 5-12 on p. 21)The CV value displayed in the "Last Blanking" field (see
Figure 13) is the coefficient of variation of all wells that were used for blanking.