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Thermo Fisher Scientific CyberScan pH 300/310 User Manual

Page 41

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8 P R O B E

CA R E

A N D

M A I NT E NA N C E

Since your pH electrode is susceptible to dirt and contamination, clean it every one to three months
depending on the extent and condition of use.

NOTE: For specialty electrode care, consult the instruction manual included with your electrode.

pH electrode storage

For best results, always keep the pH bulb wet. Use the protective electrode storage bottle or rubber
cap filled with electrode storage solution to store your electrode. Also, you can store in a pH 4 buffer
with 1/100 part of saturated KCl. Other pH buffers are OK for storage, but NEVER use distilled water
for storage.

After measuring

1.

Rinse the pH electrode and reference junction in de-ionized water.

2.

Store the electrode as recommended above in “pH electrode storage,” or as recommended by
the manufacturer.

3. Prior to next use, rinse the liquid junction with de-ionized water and tap dry – never wipe

electrode.

NOTE:

If this does not restore electrode to normal response, see “Reactivating the pH electrode”

section below.

pH electrode cleaning

Salt deposits

: Dissolve the deposits by immersing the electrode in tap water for ten

to fifteen minutes. Then thoroughly rinse with distilled water.

Oil/Grease film

: wash electrode pH bulb gently in some detergent and water. Rinse

electrode tip with distilled water or use a general purpose electrode cleaner (see the
Accessories section for ordering information).

Clogged reference junction

: Heat a diluted KCl solution to 60 to 80

°C. Place the

sensing part of the electrode into the heated solution for about 10 minutes. Allow the
electrode to cool in some unheated KCl solution.

Protein deposits

: Prepare a 1% pepsin solution in 0.1 M of HCl. Set the electrode

in the solution for five to ten minutes. Rinse the electrode with distilled water.