Jenway 6850 - Manual (Page 8)

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SECTION 1 – Introduction
1.1 INSTRUMENT DESCRIPTION
The 6850 is a variable bandwidth, double beam UV/visible spectrophotometer with an integrated user
interface for local control. This instrument has highly stable optics and two detectors that measure the
sample and reference solutions simultaneously to optimise the accuracy of the measurement. The
6850 has measurement modes for photometrics, concentration, multi-wavelength, spectrum
scanning, quantitation, kinetics and DNA and Protein analysis. The instrument is supplied with
Jenway Prism PC software to allow full PC control of the spectrophotometer.
1.2 INSTRUMENT SPECIFICATION
6850
Wavelength
Range
190 to 1100nm
Resolution 0.1nm
Accuracy ± 0.3nm (at 0.5 and 1nm bandwidth) ± 0.5nm (at 2, 4 and 5nm
bandwidth)
Repeatability ± 0.2nm
Spectral bandwidth Variable 0.5 / 1 / 2 / 4 and 5nm
Photometrics
Transmittance
0 to 200%
Absorbance -0.3 to 3.0A
Accuracy ±0.3%T (0 – 100%T), ±0.002A (0 – 0.5A)
Reproducibility ±0.001 Abs (0 to 0.5 Abs) ±0.002 Abs (0.5 to 1.0 Abs)
Resolution 0.1%T, 0.001A
Stray light <0.05% at 360nm and 220nm
Noise 0.0005A
Stability ±0.001A at 500nm after 15min warm up
Quantitation
Range
0 to 99999
Data points Up to 3 wavelengths
Calibration Blank with up to 10 standards
Units IU, mM/L, M/L, μg/mL, mg/mL, mg/L, mEq, ppb, ppm, % and other
Curve fit algorithms Linear, Linear through zero, Quadratic and Cubic
Multi-wavelength
Range
0 to 99999
Data points Up to 10 wavelengths
Kinetics
Measurement Time
Up to 12 hours
Scan Interval 0.1, 0.2, 0.5, 1, 2, 5, 10 or 30 seconds
Calibration Blank with a single standard or factor
Display Graphical and concentration
Analysis Slope and line of best fit between any two points
Spectrum
Range
Any range between 190 to 1100nm
Scan speed 100 to 2000nm/min
Scan interval Selectable 0.1, 0.2, 0.5, 1, 2 or 5nm
DNA
Measurement
modes
DNA/RNA Ratio, concentration, A320 correction