Jenway 6850 - Manual (Page 27)

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SECTION 6 – QUANTITATION
The quantitation measurement mode enables sample concentrations to be calculated using a
standard curve. In this mode a number of standard solutions covering a range of known
concentrations are measured. The absorbance or % transmittance of these solutions is plotted to
create a standard curve. Once the standard curve has been created a sample of unknown
concentration can be measured and the concentration calculated using the standard curve.
6.1 QUANTITATION MODE SCREEN
The quantitation measurement mode enables
measurement parameters to be changed. The two
options at the bottom of the screen allow the user to
select the unit of measurement and edit the calibration
standard settings. These options are selected with the
corresponding function buttons.
6.2 WAVELENGTH SELECTION
The wavelength can be adjusted by pressing the GOTO

key on the instrument keypad. The user can
select from the following options:
1. Single WL
2. Isobestic where Abs = Abs

1 – Abs 
2
3. 3 Points
The required wavelength values can then be entered using the numeric keypad. Press the Enter key
to confirm.
6.3 METHOD SETUP
In this measurement mode the method setup parameters are accessed by pressing the function keys
below the two options on the quantitation menu screen.
m x Abs λ1 + n x Absλ3
where: Abs = Abs λ2 -
m + n
m = Abs λ3 - Absλ2 and n = Absλ2 - Absλ1
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