Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 3)

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Table of Contents
1 Introduction 5
1.1 Java Script 5
1.1.1 Roboocyte2 Specific Features 5
1.1.2 Oocyte Loop 5
1.2 Variables 5
1.2.1 JavaScript Variables 5
1.2.2 User Defined Interactive Variables 6
1.2.3 Predefined Variables 6
1.2.4 Read-only Variables 7
1.3 Complete List of Commands 9
1.3.1 Conditions 9
1.3.2 Robo2. GUI-Commands 10
1.3.3 Robo2. Variable Handling Commands 10
1.3.4 Robo2. Movement Commands 11
1.3.5 Robo2. Amplifier and Data Acquisition Commands 12
1.3.6 Robo2. Data Analysis Commands 13
1.3.7 Robo2. Database Commands 14
1.3.8 Robo2. Timing Commands 15
1.3.9 Robo2. Liquid Handling (Roboflow) Commands 15
1.3.10 Robo2. High Level Commands 16
1.3.11 RecDisplay. Recording Display Commands 17
1.3.12 ControlDisplay. Control Display Command 17
1.3.13 Gilson. Gilson Commands 18
2 Example Script "Dose-Response" 19
2.1 Defining Dialogue variables 19
2.1.1 User Defined Dialogue Variables 19
2.1.2 Working with Pre-defined Variables 20
2.2 The Oocyte Loop 21
2.3 Standard Routines executed before Recording Protocol 22
2.3.1 Moving the Measuring Head into Liquid 22
2.3.2 Switching to Current Clamp Mode 23
2.3.3 Electrode Offset Compensation 23
2.3.4 Electrode Resistance Test 23
2.3.5 Oocyte Impalement 24
2.3.6 Starting Voltage Clamp Mode 25
2.3.7 Initial Leak Current Test 25
2.3.8 Final Leak Current Test with Perfusion 26
3 Recording Protocol Examples 27
3.1 Using the Dose-Response Script as a Template 27
3.2 Ligand-gated Channels and Electrogenic Transporters 27
3.2.1 Expression Test 27
3.2.2 Dose-Response Protocol 29
3.3 Voltage-gated Ion Channels 30
3.3.1 Expression Test 30
4 Using the Gilson Liquid Handler 33
4.1 Movement Commands 33
4.2 Peristaltic Pump Commands 33