Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 16)

Roboocyte2 Manual
16
1.3.10 Robo2. High Level Commands
Robo2. Parameter(s) Action Cond. Example Script
ResistanceCheck_I(a,b)
a = MIN_RESISTANCE_I
b = MAX_RESISTANCE_I
R: bool
checks if the resistance of the current
electrode is between min and max (kOhm),
returns true if this was the case
L+ HL-commands_eg.js
ResistanceCheck_U(a,b)
a = MIN_RESISTANCE_U
b = MAX_RESISTANCE_U
R: bool
checks if the resistance of the voltage
electrode is between min and max (kOhm),
returns true if this was the case
L+
HL-commands_eg.js
DCOffsetCorrection(a,b,c,d)
a = DCOFFSET_RANGE
b = DCOFFSET_DELAY
c = DCOFFSET_WAIT
d = DCOFFSET_ATTEMPTS
R: bool
checks if the offset of the I and U electrodes is
within the range given by range (mV)
delay: initial delay to wait until junction
potential is established in seconds
wait: wait time between repeats in seconds
attempts: number of repeats.
L+
HL-commands_eg.js
Impale(a,b,c,d)
a = MIN_RMP
b = IMPALEMENT_STEPS
c = IMPALEMENT_STEP
d = IMPALEMENT_WAIT
R: bool
Impalement procedure
MIN_RMP: minimum membrane potential
IMPALEMENT_STEPS: maximum number of
steps to "find" MIN_RMP on both electrodes
IMPALEMENT_STEP: single step size in µm
IMPALEMENT_WAIT: wait time in seconds
between steps
returns true if impalement was successful
W+
HL-commands_eg.js
InitialLeakCurrentCheck(a,b)
a = MIN_INITIAL_LEAKCURRENT
b = MAX_INITIAL_LEAKCURRENT
R: bool
initial leak current test, returns true if the
measured leak current is between min and max
(nA)
Oo+ HL-commands_eg.js
LeakCurrentCheck(a,b,c,d)
a = MIN_LEAKCURRENT
b = MAX_LEAKCURRENT
c = LEAKCURRENT_WAIT
d = LEAKCURRENT_ATTEMPTS
R: bool
leak current test, returns true if the measured
leak current is between min and max (nA).
Waits c seconds before determining the leak
current and retries d times
Oo+ HL-commands_eg.js