Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 29)

Appendix
29
the response can be compared to the values assigned to the user-defined variables minResponse
and maxResponse the result of which determines whether the script continues at line 297 or is
stopped and continued with the oocyte from the next well.
Example1: Negative response expected (inward current)
Condition: if (signal > minResponse || signal < maxResponse)
maxResponse = -10000
minResponse = -200
signal = -100 ==> signal > minResponse ==> next oocyte
signal = -12000 ==> signal < maxResponse ==> next oocyte
signal = -5000 ==> signal > maxResponse and signal < minResponse ==> script proceeds
Example2: Positive response expected
If the expected response is positive (outward current) operators < and > have to be exchanged.
Condition: if (signal < minResponse || signal > maxResponse)
maxResponse = 10000
minResponse = 200
signal = 100 ==> signal < minResponse ==> next oocyte
signal = 12000 ==> signal > maxResponse ==> next oocyte
signal = 5000 ==> signal < maxResponse and signal > minResponse ==> script proceeds
3.2.2 Dose-Response Protocol
After the oocyte has been tested successfully for sufficient expression and stability, the recording
protocol continues with the does-response recording part of the script. Before the dose-response
recording starts, the usual axis scaling and ROI definition commands are applied.
If the application of different agonist concentrations steps sequentially from valve a to valve a + 1
a for loop can be used.