Multichannel Systems STG4002 Manual - Manual (Page 17)

Programming Stimulus Protocols
13
5 Programming Stimulus Protocols
5.1
MC_Stimulus II Sample Files for STG 4000 Series
These sample files are intended for demonstrating basic features of the Stimulus Generator 4002,
for basic applications with the MEA System. If you are using a MEA-System, please see also the
MC_Rack tutorial in the MC_Rack help or the separate PDF on the installation volume.
The sample files are automatically installed to the Examples folder in the MC_Stimulus II program
directory.
Note: The demo files were kindly provided by STG users. MCS does not guarantee that these files
will be appropriate for your application and stimulating electrode type. For using MEA electrodes
as stimulating electrodes, MCS recommends the use of monopolar voltage pulses, though some
MEA users feel that they have better results with bipolar voltage pulses, depending on the cell
culture or tissue used.
Warning: Always obey the recommendations of the stimulating electrode manufacturer.
5.2 Neuroelectrophysiology / MEA-System
LTP-Induction.stm
 Stimulus type: Pulse train of 100 biphasic pulses for LTP induction
 Amplitude: 1.5 V
 Duration: 100 µs per phase
 Inter-Stimulus-Interval / frequency: 10 ms / 100 Hz
LTP-Testpulse.stm
 Stimulus type: Biphasic test pulse
 Amplitude: 1.5 V
 Duration: 100 µs per phase
 Inter-Stimulus-Interval: 60 s
PairedPulse.stm
 Stimulus type: Paired biphasic test pulse for paired pulse facilitation and depression
 Amplitude: 2 V
 Duration: 100 µs per phase
 Inter-Stimulus-Interval: 20 ms between paired pulses, 10 s between pairs
IOcurve-500-3000.stm
 Stimulus type: Voltage step series with biphasic pulses for I/O analysis
 Amplitude: 500 mV to 3 V, increments of 250 mV, 3 repeats
 Duration: 100 µs per phase
 Inter-Stimulus-Interval: 10 s