BARSKA Microscope - Manual (Page 10)

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Fig. 1 - Objective Parts
Adjustable
Ring Tightening
Ring Mark
Sleeve Front
Sleeve
OPERATION (cont.)
6. Adjust the interpupillary distance by using the eyepiece
interpupillary slide adjustment.
7. Observe using the right eyepiece adjusting the coarse and fine
focus and adjust the diopter ring until image is clear and sharp.
8. Observe with the left eyepiece and adjust the diopter ring until
image is clear and sharp.
9. Rotate the fine focus adjustment when using other objectives.
NOTE: This instrument is equipped with patent objectives so
the precision or parfocalization is very high.
10. If the image is in focus with the 10x objective, you can select
other objectives and observe the specimen even if the fine
adjustment knob has not been used by using the following
method (See Fig. 1):
1. Unscrew the 40x or 100x objective and remove from
turret.
2. Remove the mark sleeve.
3. Turn the ring on the objective to adjust its parfocal
distance.
4. Re-insert the objective and compare with the 10x.
5. Adjust until the 40x and 100x objectives image is clear.
USING THE CEDAR OIL
1. Drop some cedar oil on to the top of the 100x objective when the
100x objective is being used. NOTE: To maintain a good quality
image, rotate the turret right and left several times to eliminate
bubbles in the cedar oil.
2. After finishing the observation, wipe off the cedar oil.
3. Do not use the 40x objective until you have wiped off all of the
cedar oil.
OPERATION (cont.)
ADJUSTING THE CONDENSER APERTURE
1. The numerical aperture of the condenser should match the
numerical aperture of the objective being used.
2. To make sure that the objectives are imaging properly
(especially the 40x and 100x), follow this procedure:
1. Take off the eyepiece.
2. Look through the eyepiece.
3. The smallest circle or light that you can see is the
eyepiece's exit pupil.
4. Adjust the aperture of the iris diaphragm in the
condenser to 70% or 80% for the best contrast for
observation (See Fig. 2.).
Fig. 2 - Condenser Diaphram Aperture
Exit Pupil
of Objective
Aperture of
Diaphragm
TROUBLESHOOTING
Problem Possible Cause Solution
1. Image not clear. 1.Specimen is in incorrect
position.
2. Lens is dirty.
3. Cedar oil not placed on
immersion objective.
4. Bubbles in Cedar oil.
5. Cedar oil on 40x objective.
6. Iris diaphragm open too wide.
1. Re-position specimen.
2. Clean lens.
3. Put a drop of Cedar oil on
immersion objective.
4. Rotate turret several times to
eliminate bubbles.
5. Clean 40x objective.
6. Reduce size of iris diaphragm.
1. Condenser position is incorrect.
2. Lens is dirty.
3. Specimen is not placed level.
1. Re-position condenser.
2. Clean lens.
3. Re-position specimen so it is level.
1. Iris diaphragm opening too small.
2. Position of condenser too low.
3. Lens is dirty.
1. Open iris diaphragm wider.
2. Raise condenser.
3. Clean lens.
1. Specimen is in incorrect position. 1. Re-position specimen.
2. Poor illumination.
3. Illumination not bright.
4. Cannot focus at high
magnification.
1. Stage is too high. 1. Re-position stage. 5. Objective lenses touch
specimen.
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