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When to use external hardware triggering mode, Using external hardware triggering mode, Spectrasuite – Ocean Optics External Triggering Options User Manual

Page 10: Ooibase32

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External Triggering Options Instructions

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External Hardware Triggering – Trigger Timing

When to Use External Hardware Triggering Mode

Use the External Hardware Triggering mode if you:

• Are using a pulsed excitation source or light source in your experiment (such as a laser or flash

lamp)

• Are doing LIF (fluorescence with pulsed excitation) or phosphorescence experiments

• Need to synchronize an acquisition with an external event

Using External Hardware Triggering Mode

Follow the steps below to enable External Hardware Triggering mode.

Procedure

1. Supply a line from Pin 7 on the USB2000/USB2000+/Jaz//HR2000/USB4000 or Pin 10 on the

HR2000+/HR4000 of the Multi-pin Connector to your triggering device.

2. Set your acquisition parameters, such as integration time, in the software.

SpectraSuite

Use the External Trigger selection box on the Trigger toolbar to set the trigger mode of the
spectrometer to External Hardware.

OOIBase32

a. Select Spectrum | Configure Data Acquisition from the menu. Choose the External Trigger

tab and select Hardware Trigger.

b. Enable the Automatically save file on trigger box to save processed data with each external

trigger.