ChemoMetec NC-3000 - Manual (Page 47)

Appendix A: Description of changes from latest revision
991-3001 Rev. 1.3 June-2013 31
Appendix A: Description of changes from latest
revision
This chapter describes changes from latest revision of this User’s guide.
A.1 New:

N/A
A.2 Changes:

Front Page
o Revision 1.3

Page iii
o Revision 1.3
o June 2013

Inspection, Unpacking and Installation of Equipment (Page 3)
o Deleted phrase: “Also be sure to save the User’s Guide, for
instruction and reference.
”

Advanced cell analysis with the NucleoCounter
®
NC- 3000™ (Page 5)
o • Viability and Cell Count Assay
• Vitality Assay
• Mitochondrial Potential Assay

Advanced cell analysis with the NucleoCounter
®
NC- 3000™ (Page 6)
o It has 9 emission filters, and it can in the future be upgraded to a total
of 20.

Advanced cell analysis with the NucleoCounter
®
NC- 3000™ (Page 7)
o This makes the NucleoCounter ®
NC- 3000™ capable of analyzing as
much as 16µl from one sample.

Advanced cell analysis with the NucleoCounter
®
NC- 3000™ (Page 8)
o The Cassette is loaded by fully immersing the tip of the Cassette into
the sample and then gently pressing the white piston, which create s a
partial vacuum in the flow system. This results in the sample being
loaded into the flow system. Approximately 60µl is loaded into the
flow system

Advanced cell analysis with the NucleoCounter
®
NC- 3000™ (Page 9)
o During production, a calibration parameter for all Cassettes is found,
which specifies the precise depth or thickness of the measurement
chamber of the device.
o The maximal analysis volume in each chamber of a 2 chamber slide
is 16µl in an 8 chamber it is 5.6µl per chamber.

Operation of the NucleoCounter
®
(page 19)
o Figure 17 NucleoView NC- 3000™ software with a typical result
The NucleoView NC- 3000™ software is used for control of the
NucleoCounter
®
NC- 3000™ instrument and image analysis of the
recorded images. The program is installed on a Windows PC
(Windows XP SP3, Windows 7 or W indows 8).