ChemoMetec NC-3000 - Manual (Page 25)

2 Advanced cell analysis with the NucleoCounter
®
NC-3000 ™
991-3001 Rev. 1.3 June-2013 9
measurement area. The imaged area is only dependent on the optics of the
instrument. Therefore, the area is constant an d specific for each instrument.
2.2.4 Cassette handling
The image analysis method of the NucleoView NC- 3000™ offers considerable
stability, when elim inating non-cellular objects from the image, including scratches
and smears on the Cassette windows, and thus producing valid results even under
extreme conditions. On the other hand, the quality of the cell count is best assured
by keeping the windows of the Cassette as clean as possible.
Caution! In order to avoid contaminating the measurement window it is important not
to touch the window when handling the Cassettes. Be careful when attempting to
wipe off the surface of the Cassette, in an attempt to rem ove any foreign object,
since the plastic material of the Cassette can be scratched. In section 5.2 Inserting
and removing the Cassette see how to place and remove the slide from the sample
tray.
2.2.5 The slides
In order to present analysis for assays demanding external handling of reagents
traditional glass slides are available as a 2 chamber and in an 8 chamber version.
The maximal analysis volume in each chamber of a 2 chamber slide is 16 µl in an 8
chamber it is 5.6µl per chamber.
Normal pipettes can be used to load the sample to be analyzed with the slide. After
analysis the slide must be handled as biological waste. In section 5.3 Inserting and
removing the slide see how to place and remove the slide from the sample tray.
Figure 4 A two chamber slide - NC-Slide ™ A2. Note the arrows showing the
insertion direction