Hudson Robotics SoftLinx 5.0 - Manual (Page 37)

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Protocol Tutorial - Advanced
Advanced protocols require multiple steps of various types to complete. A sample advanced protocol
which utilizes the Rapid Pick is shown below.
1. Define your task.
Situation: We have a bunch of bacteria colonies in agar plates that we would like to pick, using the Rapid
Pick colony picker, and place into 96 well micro plates. The micro plates must be pre-filled with solution
from the Micro 10, and later sealed by a plate sealer of some type. We also have a plate crane to move
plates between all of the instruments.
It is highly recommended to break up this task into parts. With SoftLinx V, the user can use region activities
to label each part of the protocol separately.
This protocol has 3 parts.
A. Initial Loading of the colony picker. This portion of the protocol will only be done once.
B. Actual Colony Pick. The picker will be reloaded every time plates are needed.
C. Final unloading of the picker. This is performed once, at the end of the protocol.
For this example, the protocol requires the following interfaces:
Crane - Micro Plate Handler
Micro10 - Liquid Dispenser
RapidPick - Colony Picker
Brandel - Plate Sealer
2A. Build the Initial Plate Setup region.
We can focus on one part at a time. First, we can focus on the initial loading of the colony picker. Place a
region activity on the canvas, and label it "Initial Plate Setup".
Now, we must define what we need to do to set up the Rapid Pick for picking. Generally, preparation
should go like this:
- Move a lidded colony source plate from a stack to the RapidPick.
- Remove the lid from the source plate.
- Move a new target plate from a stack to the micro 10.