Bio-Rad VINEO™ Brettanomytest PCR Kit User Manual

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This test is used to detect and quantify Brettanomyces bruxellensis in the
DNA extracted from fermented drinks produced from grape and
fermenting grape musts.

3 - KIT CONTENTS

This kit contains the quantity of reagent required for 96 reactions.

4 - VALIDITY AND PRESERVATION
On reception, the kit must be stored at temperatures between +2°C and
+8°C. Each reagent stored between +2°C and +8°C can be used until the
expiry date indicated on the tube.
NB: Do not freeze the reagents.

5 - REQUIRED EQUIPMENT THAT IS NOT PROVIDED

Equipment:
• Industrial Diagnostic CFX96™ real-time PCR detection system, 96

wells, ref. Bio-Rad :359-3990

• CFX Manager™ Software, Industrial Diagnostic Edition, ref. Bio-Rad :

359-3893

• MiniOpticon™ system for real-time PCR, reaction block of 48 wells,

ref. Bio-Rad: 359-3995

• Vortex
• Optionally: centrifuge with rotor for plates or strips (max. 2000 x G)
• 20 μl, 200 μl and 1000 μl micropipettes
• Multi-distributor such as combitip pipettes

Note: We recommend using an uninterruptible power supply with the
thermocycler.

Stoppers

Designation

Reagent

Volume

Pink

Fluorescent probes

1 tube (0.55 mL)

White

Amplification mix

2 tubes (2 x 2.2 mL)

Green

Negative PCR control

1 tube (0.5 mL)

Red

Positive Qs PCR control

1 tube (0.5 mL)

TAKING

THE SAMPLE

DNA EXTRACTION

VINEO™ Extract

DNA Kit

(Ref.: 354-8100)

DNA

AMPLIFICATION by

PCR IN REAL TIME

VINEO™

Brettanomytest PCR

Kit (Ref.: 354-8101)

ANALYSIS &

INTERPRETATION

Opticon Monitor™

CFX Manager™ IDE