Bio-Rad VINEO™ Brettanomytest PCR Kit - Manual (Page 2)

TABLE OF CONTENTS
1 - INTRODUCTION
2 - PRINCIPLE BEHIND THE VINEO™ Brettanomytest PCR Kit
3 - KIT COMPOSITION
4 - VALIDITY AND PRESERVATION
5 - REQUIRED EQUIPMENT THAT IS NOT PROVIDED
6 - PRECAUTIONS AND RECOMMENDATIONS
7 - SAMPLING AND TRANSPORT OF SAMPLES
8 - DNA EXTRACTION
9 - REAL-TIME PCR
10 - ANALYSIS OF THE DATA
11 - INTERPRETATION OF THE RESULTS
12 - CONFIRMATION PROTOCOL FROM ISOLATED COLONIES
13 - TEST PERFORMANCE AND VALIDATIONS
APPENDIX A - PCR AMPLIFICATION REAGENT MIXTURE
PREPARATION TABLE
APPENDIX B - PLATE SETUP FOR THE Chromo4™ OR THE CFX96™
THERMOCYCLERS
APPENDIX C - PLATE SETUP FOR THE MiniOpticon™
THERMOCYCLER
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