Bio-Rad VINEO™ Brettanomytest PCR Kit User Manual
Page 44

SOMMARIO
1 - INTRODUZIONE
2 - PRINCIPIO DEL VINEO™ Brettanomytest PCR Kit
3 - COMPOSIZIONE DEL KIT
4 - PERIODO DI VALIDITÀ E CONSERVAZIONE
5 - ATTREZZATURA E MATERIALE NECESSARI NON FORNITI
6 - PRECAUZIONI E RACCOMANDAZIONI
7 - PRELIEVO E TRASPORTO DEI CAMPIONI
8 - ESTRAZIONE DEL DNA
9 - PCR IN TEMPO REALE
10 - ANALISI DEI DATI
11 - INTERPRETAZIONE DEI RISULTATI
12 - PROTOCOLLO DI CONFERMA A PARTIRE DA COLONIE ISOLATE
13 - PRESTAZIONI DEL TEST E VALIDAZIONI
ALLEGATO A - TABELLA DI PREPARAZIONE DELLA MISCELA DI
REAZIONE DI AMPLIFICAZIONE PCR
ALLEGATO B - PIANO DI PIASTRA DA UTILIZZARE SUI
TERMOCICLATORI Chromo4™ O CFX96™
ALLEGATO C - PIANO DI PIASTRA DA UTILIZZARE SUL
TERMOCICLATORE MiniOpticon™
44
© 2010 Bio-Rad
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