Bio-Rad VINEO™ Brettanomytest PCR Kit - Manual (Page 11)

11
© 2010 Bio-Rad
APPENDIX A - PCR AMPLIFICATION REAGENT MIXTURE PREPARATION TABLE
Use this table to determine the required quantities of fluorescent probes and amplification
solution to prepare the PCR reagent mixture. Calculation of the number of samples
must include the 2 positive Qs PCR controls and the negative control.
Number
of samples
Fluorescent
probes (μL)
(Pink stopper)
Amplification
solution (μL)
(White stopper)
Number
of samples
Fluorescent
probes (μL)
(Pink stopper)
Amplification
solution (μL)
(White stopper)
1 5 40 49 265 2117
2 11 86 50 270 2160
3 16 130 51 275 2203
4 22 173 52 281 2246
5 27 216 53 286 2290
6 32 259 54 292 2333
7 38 302 55 297 2376
8 43 346 56 302 2419
9 49 389 57 308 2462
10 54 432 58 313 2506
11 59 475 59 319 2549
12 65 518 60 324 2592
13 70 562 61 329 2635
14 76 605 62 335 2678
15 81 648 63 340 2722
16 86 691 64 346 2765
17 92 734 65 351 2808
18 97 778 66 356 2851
19 103 821 67 362 2894
20 108 864 68 367 2938
21 113 907 69 373 2981
22 119 950 70 378 3024
23 124 994 71 383 3067
24 130 1037 72 389 3110
25 135 1080 73 394 3154
26 140 1123 74 400 3197
27 146 1166 75 405 3240
28 151 1210 76 410 3283
29 157 1253 77 416 3326
30 162 1296 78 421 3370
31 167 1339 79 427 3413
32 173 1382 80 432 3456
33 178 1426 81 437 3499
34 184 1469 82 443 3542
35 189 1512 83 448 3586
36 194 1555 84 454 3629
37 200 1598 85 459 3672
38 205 1642 86 464 3715
39 211 1685 87 470 3758
40 216 1728 88 475 3802
41 221 1771 89 481 3845
42 227 1814 90 486 3888
43 232 1858 91 491 3931
44 238 1901 92 497 3974
45 243 1944 93 502 4018
46 248 1987 94 508 4061
47 254 2030 95 513 4104
48 259 2074 96 518 4147