Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 31)

12. Click OK. The spectrum flag changes from yellow to green for all 8 spectra, once calibration
is complete.
13. Export the data following the instructions in Appendix C and selecting the parameters for
export in the following order:
• Array bar code
• Spot name
• Substance mass
• Intensity
• Resolution
• S/N
• Peak #
14. Paste the data into the ProteinChip SELDI OQ form, data sheet “Test 4 Mass Calibration”.
Paste the data into cell V41.
15. In the Internal Table, right-click on cell B21 and select Refresh Data.
16. The spreadsheet indicates if the test passed or failed. The test passes if the following
specifications are achieved:
• Peptide average mass within 0.01% of calibrant mass
• % Standard deviation (pooled CV) of <0.01
17. If the test fails, run maintenance procedure 1 (HV conditioning) and repeat the test. Contact
technical support if the test fails a second time.
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