Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 30)

9. In the External Table, right-click on cell B3 and select Refresh Data.
10. The spreadsheet indicates if the test passed or failed. The test passes if the following
specifications are achieved:
• Peptide average mass within 0.1% of calibrant mass
• % Standard deviation (pooled CV) of <0.05
11. If the test fails, run the HV conditioning procedure (maintenance procedure 1), and repeat
the test. If the test fails a second time, contact technical support.
Mass Accuracy — Internal Calibration Test
Perform this test AFTER the external calibration test (above).
1. Check that all peaks have been selected and that all peaks are marked correctly (not on
shoulders, etc.).
2. Select all 8 spectra.
3. Select Spectra > Calibrate > Apply Calibration Protocol.
4. Select OQ Mass Cal.
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