Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 13)

1. Insert the ProteinChip detector calibration array into the instrument.
Note: For the Enterprise Edition instrument, place the array into slot 1 of a cassette and fill the
rest of the slots with blank or used ProteinChip arrays.
2. Open the instrument’s interactive web page (http://pcs4000-####/index.jsp, where #### is
the instrument serial number). If the instrument is under local control, the web page will only
be on the local computer. If it is installed on an intranet, it will be available on all intranet
computers.
3. Select Calibrations > Automatic Detector Gain. The Detector Gain page opens. Check
that Automatic and NOT manual is selected.
4. If using the Enterprise Edition instrument, select Array number 1 (slot 1 in cassette).
5. If this is the first calibration performed on the instrument, or if the last calibration was performed
more than two weeks ago, select two previously unused spots. If this is a routine, weekly
calibration, select one unused spot.
6. Click Start.
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