Bio-Rad ProteinChip Qualification and Calibration Kits User Manual
Page 26
7. Plot the peaks with the filtering option turned off:
a.
Select all 8 spectra.
b.
Click the data analysis icon
. The Analysis Settings dialog opens.
c.
Open the Filtering tab and deselect the On checkbox.
d.
Click OK.
8. Plot all 8 spectra. Using the peak selection tool, select all 7 peaks. Each spectrum should
resemble that shown below (intensities relative to each other may vary). Additional peaks
other than those indicated may consist of matrix, doubly-charged peaks, dimers or other
multimers, salt peaks, etc., as for any mass spectrum.
Peak
Name
Molecular Weight (Da)
1
Arg-8-vasopressin
1084.247
2
Somatostatin
1637.903
3
Dynorphin A
2147.5
4
ACTH
2933.5
5
Beta endorphin
3465
6
Arg-insulin
5963.8
7
Cytochrome C
12230.92
23
10010680A.qxp 8/17/2007 1:58 PM Page 23
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