Bio-Rad ProteinChip Data Manager Software - Manual (Page 7)

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ProteinChip SELDI System: Reader Guide
Mass Resolving Power
Peptide
Better than 1000 for [Arg 8 ]-vasopressin at 1084 Da, standard operating voltage
Mass Accuracy
Inherent
Measured mass will be within 0.5% of true mass with 95% confidence
(0.25% CV)
External calibration
Measured mass will be within 0.1% of true mass with 95% confidence
(0.05% CV)
Internal calibration
Measured mass will be within 0.01% of true mass with 95% confidence
(0.005% CV)
Laser
Pulsed nitrogen laser (337 nm), 4 ns pulse width
Sample Handling
Personal
Runs one 8-spot array within 20 minutes using 250 transients per spot
Enterprise
Runs a cassette of up to twelve 8-spot ProteinChip arrays within 2 hours using
250 transients per spot
Vacuum System
Operating pressure < 2.7 x 10 -4 Pa
Gauges: active inverted magnetron (high vacuum) and active Pirani (low
vacuum) turbomolecular pump