Bio-Rad ProteinChip Data Manager Software - Manual (Page 19)

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ProteinChip SELDI System: Reader Guide
Multicassette Autoloader (Enterprise Edition)
The ProteinChip SELDI system Enterprise Edition comes with a multicassette
autoloader. It contains cassette loader and unloader assemblies designed to
allow the automated processing of multiple cassettes from a stack. The purpose
of this feature is to allow unattended operation of the reader for significant
periods of time. The loader processes the cassettes sequentially, removing each
cassette from an input stack, cycling the designated arrays in the cassette
through the load lock and source chamber, then transferring the processed
cassette to the output unloader before returning to collect the next cassette. This
system is designed for continuous unattended processing of up to 14 cassettes
in one run. A maximum of 14 cassettes can be loaded into the stacker at one
time. The cassette lids must be closed in order for the reader to run.
Overview of Operation
The following instructions are intended to be a brief guide only. For more
information on data acquisition and analysis, please consult the ProteinChip data
manager software manual.
Acquiring control of the reader
The status of the sensors is displayed in the Home tab of the software interface
for the reader (refer to Fig. 12). The sensors should have the following status
before beginning operation:
Enterprise Edition
• Vacuum state — operating
• High voltage — off
• Cassette — present
• Loader cover — closed
• Unloader cover — closed
• High vacuum — <2.7 x 10 -6 Pa.
Control of the reader is obtained by clicking the Start icon.
Personal Edition
• High voltage — off
• Door — closed
• Lock — locked
Control of the reader is obtained by clicking the Start icon.