Bio-Rad AG® MP-1M Anion Exchange Resins User Manual
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batch as well as column applications. Medium mesh is
an ideal, general purpose particle size for use in analyti-
cal and preparative scale column chromatography. Fine
mesh material (200-400 and minus 400 mesh) is used
for high resolution analytical separations.
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The AG 1 and AG MP-1 resins are available in sev-
eral particle size ranges. The flow rate in a chromato-
graphic column increases with increasing particle size.
However, the attainable resolution increases with
decreasing particle size and narrower size distribution
ranges. Particle size is given either in mesh size or
micron size. The larger the mesh size number, the small-
er the particle size. Table 4 shows wet mesh and equiva-
lent micron diameters.
Table 4. Wet Mesh and Equivalent
Micron Diameters
Wet Mesh
(U.S. Standard)
16
20
40
50
80
100
140
200
270
325
400
Micron Diameter
(1 µm = 0.001 mm)
1,180
850
425
300
180
150
106
75
53
45
38
Large mesh material (20-50 and 50-100 mesh) is
used primarily for preparative applications and batch
operations where the resin and sample are slurried
together. Medium mesh resin (100-200) may be used in
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