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Bio-Rad AG® MP-1M Anion Exchange Resins User Manual

Page 16

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Recommended Eluant and

Metals

Resin

Eluted Ions

Reference

Bi, Pb, Cd,

Ag 1-X8

Pb, Cd, Zn -

Strelow, F. W. E.,

Zn

resin

HBr-HNO

3

;

Anal. Chem., 50, 1359

Bi - EDTA

(1978).

Table 9. Peptide and Protein Separations
on Anion Exchangers

Application

Resin

Reference

Separation of small

AG 1-X2

Titani, K., Koide, A., Ericsson,

peptides from rabbit

resin

L. H., et al., Biochem., 17, 5680

muscle

(1978).

Separation of peptides

AG 1-X2

Ozuls, J., Craig, G. and Nobrega,

from horse liver cyto-

resin

F. G., J. Biol. Chem., 251, 6767

chromes

(1976).

Purification of fungal

AG 1-X4

Bhella, R. S. and Altosaar, I., Anal.

glucoamylase

resin

Biochem., 140, 200 (1984).

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Recommended Eluant and

Metals

Resin

Eluted Ions

Reference

Ni, Co, Cu, AG 1-X8

Ni - 96% MeOH, Fritz, J. S. Pietrzyk,

Zn

resin

0.2 M HCl; Co -

D. J., Talanta, 8, 143

55% IPA, 1.3 M

(1961).

HCl; Cu - 55%
IPA, 0.1 M HCl;
Zn - 0.005 M HCl

Mn, Co, Ni

AG 1-X8

Mn, Co, Ni - 8.5

Morie, G. P., and

Fe, Mo,

resin

x 10

-2

M tartrate;

Sweet, T. R., J.

(also Cr, Zn,

Fe - tartaric acid

Chromatog., 16, 201

Cd, Hg)

in 0.1 M HCl;

(1964).

Mo - 3 M NaOH

Th, Hf, Zr,

AG 1-X8

Th - 0.7 N

Strelow, F. W. E. and

Mo

resin

H

2

SO

4

; Hf - 1.25

Bothma, C. J. C.,

N H

2

SO

4

; Zr -

Anal. Chem., 39, 595

2.0 N H

2

SO

4

; (1967).

Mo - 2.0 N NH

4

;

NO

3

, 0.5 N NH

3

V, Th, Fe

AG 1-X8

Absorbed as

Korkisch, J. and

resin

citrate com-

Krivanec, H., Anal.

plexes; Th - 8 M

Chim. Acta, 83, 111

HCl; Fe - IBMK, (1976).
acetone, 1 N HCl
(1:8:1 v/v);
V - 1 M HCl

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