Bio-Rad AG® MP-1M Anion Exchange Resins - Manual (Page 14)

Section 7
Applications
Strong anion exchange resins are used for sample
preparation, enzyme assays, metal separations, and pep-
tide, protein, and nucleic acid separations. The tables
below summarize the applications.
Table 7. Anion Exchange Resins for
Sample Preparation
Application Resin Reference
Recovery of P
i
from AG 1-X4 Stroop, S. D. and Boyer, P. D.,
glucose-phosphate resin Biochem., 24, 6, 2304 (1985).
Extraction of AG 1-X8 Dombro, R. S. and Hutson, D. G.,
5-hydroxy-indole resin Clin. Chim. Acta, 100, 231 (1980).
acetic acid from
CSF and urine
Anion removal from AG 1-X8 Torben, K. and Penderson, J. S.,
porphyrin in urine resin Scand. J. Clin. Lab. Invest., 38, 279
(1978).
Purification of cyclic AG 1-X8 Shanfield, J., Jones, J. and
nucleotides resin Davidovitch, Z., Anal. Biochem.,
113, 256 (1981).
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Ames Keta-Diastix test for glucose
1 M NaCl
Procedure
1. Pack the Poly-Prep chromatography column with
2 ml AG 1-X8 resin, 200-400 mesh, formate form.
2. Convert the resin to the OH form by washing it with
10 bed volumes of 1 N NaOh, then with 5 bed vol-
umes of distilled water.
3. Add 1 ml of the glucose sample.
4. Wash with 3 bed volumes of water.
5. Check for the presence of glucose.
6. Elute the glucose with 1 M NaCl.
7. Check for glucose.
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LIT212C 6/17/98 12:25 PM Page 22