Bio-Rad AG® MP-1M Anion Exchange Resins - Manual (Page 12)

2, Trail Edition, Laboratory Manual of the Chemical
Education Material Study.
Materials
AG 1-X8 resin, 50-100 mesh, 10 grams
Glass column approximately 12 mm ID, 30-40 cm
long, and resistant to 9 N HCl
HCl approximately 9 N, 5 N, and 0.5 N
Sample solution - 0.1 meq Co 2+ , Ni 2+ , and Fe 3+ pre-
pared from 238 mg CoCl
2
·6H
2
O; 238 mg NiCl
2
·6H
2
O;
and 271 mg FeCl
3
·6H
2
O in 10 ml 9 N HCl
Cobalt test solution - 10% NH
4
SCN in acetone
Nickel test solution - 1% KSCN or 1% NH
4
SCN
Test tubes or vials, 12
Procedure
1. Slurry the resin in distilled water.
2. Pour the resin into a column that is resistant to 9 N
HCl. The resin bed should be approximately 10 cm
deep.
19
Table 6. Suggested Flow Rates for
Ion Exchange Resin Columns
Linear
Flow Rate
Application (cm/min)
Removing trace ions 5-10
Separations with very few components 1-3
Separations of multi-component samples 0.3-1.0
Using high resolution resins
with small particle size
1-2
Section 6
Sample Protocols for
Anion Exchange Resins
6.1 Separation of Metal Ions
This experiment was suggested by Professor Harold
Walton, University of Colorado, and Professors Charles
Koch and George Pimental, University of California at
Berkeley. It is a modification of Experiment 32, Volume
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LIT212C 6/17/98 12:25 PM Page 18