Bio-Rad AG® MP-1M Anion Exchange Resins - Manual (Page 13)

12. Observe and record the colors in the resin bed and in
the eluant.
13. Test each aliquot for each of three ions:
Co +2 test - 1 drop eluant plus 1 drop cobalt test solu-
tion. Strong test is a bright blue color.
Ni +2 test - 1 drop eluant plus 1 drop nickel test solu-
tion. Neutralize with concentrated NH
3
. A bright red
ppt is a positive test for nickel.
Fe +3 test - 1 drop eluant plus 1 drop iron test solu-
tion. A bright red color is a positive test.
6.2 Glucose Binding on AG 1-X8 Resin
This procedure demonstrates the binding and elution
of glucose on AG 1-X8 resin.
Materials
Poly-Prep ® disposable chromatography column, 2 ml
1 N NaOH
AG 1-X8 resin, 200-400 mesh, formate form, 2-3 ml
Glucose sample, 100 mg/ml
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3. Equilibrate the resin by passing approximately 15 ml
of 9 N HCl over the resin bed.
4. Adjust the flow rate to no faster than 1 drop/second
or 4 ml/min.
5. When the level of 9 N HCl has reached the top of
the resin bed, either shut the screw clamp, or add 2
ml of sample (0.2 meq of each ion).
6. Do not allow air into the resin bed because air may
cause channeling with uneven flow of subsequent
solutions.
7. After the sample has soaked into the resin bed, add
approximately 20 ml of 9 N HCl.
8. Begin collecting 5 ml aliquots.
9. The color of the eluant should intensify and then the
eluant should become nearly colorless in the third or
fourth aliquot.
10. Continue elution using 20 ml of 5 N HCl, and then
20 ml of 0.5 N HCl, in the manner described above.
11. In each case, 5 to 10 ml more of the eluant may be
added if the eluant is still strongly colored after 20
ml of acid has soaked into the resin bed.
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LIT212C 6/17/98 12:25 PM Page 20