Bio-Rad Profinity eXact™ Purification and Tag Cleavage Consumables - Manual (Page 8)

Section 4
Connection to Medium
and High-Pressure
Chromatography Systems
Bio-Scale Mini cartridges can be connected to any
liquid chromatography system, provided that the
system does not exceed the maximum pressure
limit of the cartridges (3 bar, 45 psi or 300 kPa). It is
recommended that the system pressure limit be set
according to the cartridge pressure limit. Pressures
in excess of 3 bar are usually caused by restrictions
in tubing or detector cells downstream from the
cartridge. Bio-Rad offers two fitting kits for easy
connection of a Bio-Scale Mini cartridge to a
BioLogic DuoFlow
™ , HPLC-, or FPLC-type system.
4.1 BioLogic DuoFlow Systems
The luer to BioLogic system fittings kit (catalog
#732-0113) includes 1/4-28 female to male luer
and 1/4-28 female to female luer to connect one
Bio-Scale Mini cartridge to the BioLogic DuoFlow
system. (see Figure 4)
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4. Connect the cartridge outlet to the 1.6 mm ID
tubing leading to the BioLogic LP system
optics module or to the Model EM-1 Econo UV
monitor. It is recommended to use the shortest
length (approximately 10 cm) of 1.6 mm ID
tubing. Connect a barb to female luer to the
1.6 mm ID tubing, then connect to the bottom
of the male luer on the Bio-Scale Mini cartridge.
Fig. 3. Cartridge and fittings. Luer fittings and column: a
cartridge should be mounted vertically with the arrow on the
cartridge pointing downward.
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