Bio-Rad Profinity eXact™ Purification and Tag Cleavage Consumables - Manual (Page 2)

Table of Contents
Section 1 ... Introduction ........................................1
Section 2 Product Information............................2
Section 3 Connection to Low-Pressure
Chromatography Systems ..................7
Section 4 Connection to Medium- and High-
Pressure Chromatography Systems .11
4.1 BioLogic DuoFlow™
Systems .................................11
4.2 HPLC Systems .......................12
4.3 FPLC Systems........................13
Section 5 ... Buffers and Methods ........................14
Section 6 Quick Solubility Screening
Protocol............................................16
Section 7 Preparation of E. coli Lysate .............19
Section 8 Cartridge Preparation and
Purification Protocol .........................20
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10011164 Rev B
10011164A.qxp 11/19/2007 9:54 AM Page II