Bio-Rad Profinity eXact™ Purification and Tag Cleavage Consumables - Manual (Page 5)

Table 3. Buffer and chemical compatibilities for
Profinity eXact cartridges.
Reagent
Group
Description Stability
Triggering Ions F-, Cl-, N
3
-, NO
2
-, CO
2
- Do not use in lysis and
wash buffers.
See Table 5 for use as
trigger in elution buffer.
Salts Sodium Acetate £3M
NaCl or KCl Do not use in lysis and
wash buffers.
Buffers Tris-HCl Substitute Tris-acetate
or Tris-phosphate.
Acids HCl Do not use. Substitute
acetic or phosphoric
acid.
Detergents Non-ionic £5% (w/v)
Zwitterionic £5% (w/v)
Ionic Do not use.
Protease PMSF, CalBioChem cocktail,
inhibitors Roche Protease Inhibitor 1X
cOmplete mini tablet
BD Biosciences cocktail 2X
Lysis Solutions Lysis & Extractrion Reagent 1X
(Bio-Rad), B-PER phosphate
(Pierce), BugBuster (EMD),
FastBreak Cell Lysis (Promega)
ReadyPreps Lysis (Epicentre), Do not use.
CelLytic Express (Sigma)
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Table 2. Profinity eXact resin specifications.
Functional ligand Mutant subtilisin
Base bead Superflow 6% agarose
Particle size range 60–160 µm
Recommended linear flow rate <1,000 cm/hr at 25°C
Maximum operating pressure 45 psi
Chemical compatibility See Table 3
Storage 4°C
Shelf life in storage buffer >1 year at 4°C
Operational temperature 4–40°C
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