Bio-Rad Profinity eXact™ Purification and Tag Cleavage Consumables User Manual

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Note: The resin may also be cleaned with
0.1 M NaOH. Limit exposure time to 3 hours
and immediately neutralize with 5 CV of
bind/wash buffer. Regeneration of the resin
with phosphoric acid is still necessary, as
NaOH alone will not efficiently remove the
Profinity eXact tag from the resin.

cartridge after five purifications, however, the following
regeneration procedure may be used to prolong the
useful lifespan of a cartridge. In addition, in order to
avoid cross-contamination it is recommended that
single cartridges be designated for single proteins.
In order to reuse a cartridge, the Profinity eXact tag,
which is bound tightly to the functional ligand,
MUST be removed. Therefore, to maintain good
cartridge flow properties, and to prepare the cartridge
for a subsequent purification, it is recommended
that the cartridge be regenerated after each use.
For the 1 ml cartridges, run the regeneration protocol
at 3 ml/min. For the 5 ml cartridges, run the
regeneration protocol at 10 ml/min.

Regeneration and Storage

1. After elution of the purified protein, wash the

cartridge with 5 CV of bind/wash buffer.

2. Regenerate the cartridge with 3 CV of 100 mM

phosphoric acid.

3. Neutralize the cartridge with 5 CV of bind/wash

buffer.

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