Bio-Rad Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges - Manual (Page 19)

5.1 With a Liquid Chromatography System
1. Set the flow rate to the desired flow.
a. 10 ml cartridge: 0.5–4.0 ml/min at room
temperature, 0.5–2.0 ml/min at cold room
temperature.
b. 50 ml cartridge: 2.5–20 ml/min at room
temperature, 2.5–10 ml/min at cold room
temperature.
2. Wash the prepared cartridge with degassed
buffer as indicated below.
a.
10 ml cartridge: 10 ml
b. 50 ml cartridge: 50 ml
3. Inject up to the amount of sample indicated
below, discarding the first amount of effluent
indicated below.
a.
10 ml cartridge: 6 ml of sample, discarding
the first 3 ml of effluent
b. 50 ml cartridge: 30 ml of sample, discarding
first 15 ml of effluent
4. Monitor the cartridge effluent with a UV monitor
or conductivity monitor.
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