Bio-Rad Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges - Manual (Page 18)

Section 5
Desalting and Buffer
Exchange
Samples can be desalted or the sample buffer
exchanged by connecting the cartridge to a liquid
chromatography system. The void volume for the 10
ml Bio-Scale Mini Bio-Gel P-6 Desalting cartridge is
3.6 ml and for the 50 ml Bio-Scale Mini Bio-Gel P-6
Desalting cartridge is 18 ml. With the 10 ml
cartridge the desalted sample, the sample, or the
sample in its new buffer is contained within the
fraction between 3 ml and 9 ml with the highest
concentration of sample in the 3.6 to 6.0 ml fraction.
With the 50 ml cartridge the desalted sample or the
sample or the sample in its new buffer is contained
within the fraction between 15 ml and 45 ml with
the highest concentration of sample in the 18 to
30 ml fraction.
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