Bio-Rad Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges - Manual (Page 16)

Section 4
Preparing a Cartridge
For Use
Bio-Scale Mini Bio-Gel P-6 cartridges contain
20 mM Bis-Tris, pH 6.5 + 0.05% sodium azide as
the storage solution. The fully hydrated support is
ready to use after equilibrating the cartridge in the
buffer of choice. To perform a buffer exchange,
connect the cartridge to a liquid chromatography
system or peristaltic pump and condition it as
instructed below:
1. Set the pump flow rate for the 10 ml cartridge
to 4.0 ml/min (192 cm/hr) for room temperature
use or 2.0 ml/min (96 cm/hr) for cold room
temperature use.
2. Set the pump flow rate for the 50 ml cartridge to
20.0 ml/min (192 cm/hr) for room temperature
use or 10.0 ml/min (96 cm/hr) for cold room
temperature use.
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