Bio-Rad Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges - Manual (Page 17)

3. Equilibrate the cartridge with 2.2 column volumes
of degassed equilibration buffer: 6 minutes at the
room temperature flow rate setting or 12 minutes
at the cold room temperature setting.
4. Reduce the flow rate to that which will be used
in the buffer exchange or desalting cycle.
4.1 Sample Preparation
If the sample contains insoluble material, filter it with
a 0.45 µm filter before applying it to the cartridge.
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