Bio-Rad UNOsphere™ Rapid S Media - Manual (Page 8)

Table 2 UNOsphere Q, S, and rapid S
Specifications
UNOsphere Q UNOsphere S UNOsphere Rapid S
Type of ion exchanger Strong anion Strong cation Strong cation
Functional group -N+(CH
3
)
3
-SO
3
-SO
3
Total ionic capacity 120 µeq/ml Ni 2+ 260 µeq/ml 140 µeq/ml
Dynamic binding capacity*
At 150 cm/hr 180 mg BSA/ml 60 mg IgG/ml 60 mg/ml
At 600 cm/hr 125 mg BSA/ml 30 mg IgG/ml 30 mg/ml
Shipping counterion Cl- Na+ Na+
Median particle size 120 µm 80 µm 100 µm
Recommended 50–1,200 cm/hr 50–1,200 cm/hr 50–800 cm/hr
linear flow rate
Chemical stability
1.0 M NaOH (20ºC) Up to 10,000 hr Up to 10,000 hr Up to 800 hr
1.0 M HCl (20ºC) Up to 200 hr Up to 200 hr Up to 200 hr
pH stability 1–14 1–14 1–14
Antimicrobial agent 20% ethanol 20% ethanol 20% ethanol
Regeneration 1–2 M NaCl 1–2 M NaCl 1–2 M NaCl
Storage conditions 20% ethanol or 20% ethanol or 20% ethanol or
0.1 M NaOH 0.1 M NaOH 0.1 M NaOH
* 10% breakthrough capacity determined with 4.5 mg/ml human IgG (UNOsphere
Rapid S) and 2.0 mg/ml BSA (UNOsphere Q) in a 1.1 x 20 cm column.
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