Bio-Rad UNOsphere™ Rapid S Media - Manual (Page 23)

Section 5
Cleaning-in-Place (CIP) and
Sanitation
If a column no longer yields reproducible results, the
media may require thorough CIP and sanitation to
remove strongly bound contaminants. Acceptable
CIP agents include 25% acetic acid, 8 M urea, 1%
Triton X-100, 6 M potassium thiocyanate, 70%
ethanol, 30% isopropyl alcohol, 1 N HCl, 1 N
NaOH, and 6 M guanidine hydrochloride.
1. Sanitize the support in the column with 2–4 bed
volumes of 1.0 M NaOH at 50–100 cm/hr while
maintaining a minimum contact time of 40 min.
2. To reequilibrate the column, wash the column
with 2–4 bed volumes of 0.5–2 M NaCl solution
(containing 50–100 mM buffer).
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