Bio-Rad UNOsphere™ Rapid S Media - Manual (Page 18)

4. Equilibrate the cartridge with low-salt buffer for
5 min.
5. Reduce the flow rate to the rate that will be used
in the purification protocol.
4.1 Sample Preparation
Proper pH and ionic strength is necessary for
consistent and reproducible results. Sample can be
exchanged into the starting buffer or diluted to the
starting buffer concentration. This can be achieved
by diluting the sample to the ionic strength of the
starting buffer, dialyzing against the starting buffer,
or exchanging it into the starting buffer. Buffer
exchange can be accomplished using the Bio-Scale
Mini P6 cartridge, Bio-Spin
® 6 or Bio-Spin 30
columns, Econo-Pac ® 10DG desalting columns, or
Bio-Gel ® P-6DG gel filtration gel. The choice of
product will depend on sample volume. All samples
should be filtered through a 0.45 µm filter prior to
cartridge application.
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