Bio-Rad UNOsphere™ Rapid S Media User Manual
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Table 3 Common Buffers for Ion Exchange
Chromatography.
1,2,3
Type of Buffering
Cation
Ion Exchanger Buffer Range
Acetic acid
4.8–5.2
Citric acid
4.2–5.2
HEPES 7.6–8.2
Lactic acid
3.6–4.3
MES 5.5–6.7
MOPS 6.5–7.9
Phosphate 6.7–7.6
PIPES 6.1–7.5
Pivalic acid
4.7–5.4
TES 7.2–7.8
Tricine 7.8–8.9
Anion
Bicine 7.6–9.0
Bis-Tris 5.8–7.2
Diethanolamine 8.4–8.8
Diethylamine 9.5–11.5
L-Histidine
5.5–6.0
Imidazole 6.6–7.1
Pyridine 4.9–5.6
Tricine 7.8–8.9
Triethanolamine 7.3–8.0
Tris 7.5–8.0
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