Bio-Rad Nonmagnetic Beads and Related Reagents - Manual (Page 32)

9. Select Step 7 Run Protocol and select 50 beads per region with a
sample size of 50 µl.
10. If analyzing the assay at the high PMT setting, ensure that this setting
is selected prior to the run.
Analyzing Coupled Beads
1. Ensure the presence of sufficient sheath fluid and empty the waste
bottle.
2. Shake the plate at 1,100 rpm for 30 sec immediately before starting
the run.
3. Remove sealing tape and any plate cover before placing the plate on
the Bio-Plex system.
4. Select Start in the Run Protocol dialog to initiate the run.
5. When data acquisition is complete, select Shut Down from the
toolbar and follow the instructions to shut the reader down.
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