Bio-Rad Nonmagnetic Beads and Related Reagents - Manual (Page 22)

Section 8
Oligonucleotide Buffers
20
Buffers
0.1 MES, pH 4.5
(Coupling Buffer)
0.02% Tween-20
(Wash Buffer I)
0.1% SDS
(Wash Buffer II)
TE, pH 8.0
(Sample Diluent)
1.5 X TMAC
Hybridization Solution
(Microsphere Diluent)
1 X TMAC
Hybridization Solution
(Detection Buffer)
6X SSPET
(Stringent Wash Buffer)
Reagent Catalog Number Final Concentration Amount
MES (2[N-Morpholino]
ethanesulfonic acid)
5 N NaOH
Filter Sterilize and store at 4°C
TWEEN® 20
(Polyoxyethylenesorbitan
monolaurate)
Filter Sterilize and store at ambient
SDS (Sodium lauryl
sulfate) 10% solution
Filter Sterilize and store at ambient
Tris-EDTA Buffer,
pH 8.0, 100X
Filter Sterilize and store at ambient
5 M TMAC
20% Sarkosyl solution
1 M Tris-HCl, pH 8.0
0.5 M EDTA, pH 8.0
Store at ambient
5 M TMAC
20% Sarkosyl solution
1 M Tris-HCl, pH 8.0
0.5 M EDTA, pH 8.0
H
2
0
Store at ambient
SSPE, 20X
(Phosphate buffer,
pH 7.4, sodium
chloride, EDTA)
Triton® X-100
Filter Sterilize and store at ambient
Sigma M2933
Fisher SS256-500
Sigma P9416
Sigma L4522
Sigma T9285
Sigma T3411
Sigma L7414
Sigma T3038
Invitrogen 15575-020
Sigma T3411
Sigma L7414
Sigma T3038
Invitrogen 15575-020
----
Sigma S2015
Sigma T9284
0.1 M
----
0.02%
0.10%
1X
4.5 M
0.15%
75 mM
4 mM
3 M
0.10%
50 mM
4 mM
----
6X Concentrate
0.005%-0.01%
4.88 g
~ 5 drops
50 μL
2.5 mL
2.5 mL
225 mL
1.88 mL
18.75 mL
3.0 mL
3.0 mL
1.25 mL
12.5 mL
2 mL
84.25 mL
75 mL
12.5-25 μL