Bio-Rad Nonmagnetic Beads and Related Reagents - Manual (Page 29)

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4. Centrifuge the tubes at 14,000 x g for 4 min. Carefully remove and
discard the supernatant. Dilute the streptavidin-PE to 2 µg/ml with
staining buffer. Add 50 µl of the diluted streptavidin-PE to the only
tube labeled “test”. Add 50 µl of staining buffer to the negative control
tube. Do not add streptavidin-PE to the negative control tube. Cover
the tubes with aluminum foil and incubate at room temperature for 10
min without rotation.
5. Resuspend the pellet in 125 µl of storage buffer. Vortex the beads at
medium speed for 15 sec. Transfer each 125 µl sample to a single
well of a flat-bottom 96-well plate. Proceed to Section 11 to read the
coupled beads.