Bio-Rad Bio-Plex® Precision Pro™ Human Cytokine Assays User Manual

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Acquire Data
1. Shake the assay plate at 1,100 rpm for 30 sec immediately before

acquiring data. Failure to do so will result in increased data acquisition
time due to bead settling.

2. Check that the filter plate is flat. While pressing on one end of the

plate, observe the distance that the opposite end of the plate is
raised off a flat surface. If the distance is >1 mm, transfer all contents
to a flat-bottom 96-well plate or another filter plate.

3. Visually inspect the plate and ensure that the assay wells are filled with

buffer prior to placing the plate in the Bio-Plex microplate platform.

4. Slowly remove the sealing tape and any plate cover before placing

the plate in the reader.

5. Select Step 7 (Run Protocol):

a) Specify data acquisition for

100 beads per region.

b) In Advanced Settings, set the Bead Map to

25 region.

NOTE: Bio-Plex Precision Pro cytokine assays contain magnetic
beads and require the use of the 25 region map available in Bio-
Plex Manager software version 4.1 or higher.

c) In Advanced Settings, set the sample size to

50 µl.

d) In Advanced Settings, confirm that the default DD gate values

are set to

5000 (low) and 32000 (high).

NOTE: When using a Luminex instrument, set the gates
according to the Luminex procedure located in the manual.

e) Select Start and save the .rbx file. Then follow the instructions for

data acquisition.