Bio-Rad Bio-Plex® Precision Pro™ Human Cytokine Assays - Manual (Page 17)

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Section 9
Data Acquisition
Bio-Plex ® Precision Pro™ cytokine assays require the use of Bio-Plex
Manager™ software version 4.1 or higher. Recommendations for acquiring
data using the Bio-Plex suspension array system are listed below.
Alternatively, refer to the Bio-Plex Manager™ software user guide or the
instructions provided with the Luminex instrument.
Prepare System
1. Empty the waste bottle and fill the sheath fluid bottle before starting
(if HTF not present). This will prevent fluidic system backup and
potential data loss.
2. Turn on the reader and microplate platform (and HTF if present). Allow
the system to warm up for 30 min.
3. Select Start up and follow the instructions to prepare the reader
to acquire data. If the system is idle for 4 hr, the lasers will automatically
turn off and a 30 min warm-up period will again be required prior to
acquiring data. Select Warm up and wait for the optics to reach
operational temperature.
Calibrate With High RP1 Target Value
Calibrate using Bio-Plex calibration beads and target values. Daily
calibration is recommended before acquiring data.
1. Select Calibrate and confirm that the default values for CAL1
and CAL2 are the same as the values on the Bio-Plex calibration
bead labels. Use the Bio-Plex High RP1 target value for CAL2
calibration for Bio-Plex Precision Pro cytokine assays.
NOTE: When acquiring data for Bio-Plex Precision Pro cytokine
assays with a Luminex instrument, Luminex software, and Luminex
calibration beads, it is necessary to convert the Luminex CAL2
calibration bead RP1 target value using the following equation:
Bio-Plex High RP1 target value = (Luminex RP1 target value) x 4.55