Bio-Rad Human MMP and TIMP Assays - Manual (Page 8)

6
Prewet wells
(for filter plate only)
Add 50 μl 1x beads to wells
Wash 2 x 100 μl
Add 50 μl standards, samples, controls;
incubate on shaker at 850 rpm for 1 hr at RT
Wash 3 x 100 μl
Add 25 μl 1x detection antibody; incubate
on shaker at 850 rpm for 30 min at RT
Wash 3 x 100 μl
Add 50 μl 1x streptavidin-PE; incubate
on shaker at 850 rpm for 10 min at RT
Wash 3 x 100 μl
Resuspend in 125 μl assay buffer;
shake at 850 rpm for 30 sec
Acquire data on Bio-Plex system
Assay Workflow
Notes on sample preparation: Once thawed, keep samples on ice.
Prepare dilutions just prior to the start of the assay and equilibrate to room
temperature before use.