Bio-Rad Human MMP and TIMP Assays User Manual

Page 27

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b. Enter a dilution factor of 3 and click Calculate. The concentrations

for each standard point will be populated for all analytes in the table.

c. Optional: enter the lot number of the vial of standards into the

Standard Lot box and click Save.

5. Click Enter Controls Info.
a. For user-specified controls, select an analyte from the dropdown

menu, then enter a description and concentration. Repeat for
each additional analyte in the assay.

For the quality controls supplied with the premixed panel
only, format the appropriate wells as controls, enter descriptions
but leave the concentrations blank. Alternatively, the quality
controls can be formatted as samples with clear descriptions
such as “quality control.” In any case, the expected control
ranges provided are not entered into Bio-Plex Manager
software version 6.1 and earlier.

Fig. 4. Plate formatting.