Bio-Rad Human MMP and TIMP Assays - Manual (Page 2)

Table of Contents
Introduction 1
Principle 2
Kit Contents and Storage 4
Recommended Materials 5
Assay Workflow 6
lmportant Considerations 7
Detailed Instructions 7
1.
1. Plan Plate Layout 8
2.
2. Prepare Instrument 9
3.
3. Prepare Wash Method 10
4.
4. Preparing Buffer and Diluent 11
5
5. Prepare Standards and Controls 11
6. Prepare Samples 13
7.
7. Prepare Coupled Beads 17
8
8. Run Assay 18
9
9. Read Plate 22
Troubleshooting Guide 29
Plate Layout Template 34
Calculation Worksheet 35
Safety Considerations 37
Legal Notices 37
Ordering Information 38