Bio-Rad Experion Protein Analysis Kits - Manual (Page 8)

Experion RNA StdSens Starter Kit Tests
Test Name Purpose Description
1 RNA analysis basics Used for familiarization Run an Experion RNA StdSens
with the Experion system analysis of 12 replicates of a total RNA
and for troubleshooting. sample (100 ng/µl), check the resulting
Demonstrates the basic electropherograms for performance,
features, functions, and and determine the reproducibility of
reproducibility of an quantitation
Experion RNA StdSens
assay
2 Quantitation across a Used for demonstration of Prepare a dilution series of the total
range of the quantitative dynamic RNA sample (25–500 ng/µl) and use
concentrations range of the Experion RNA this series to analyze the reproducibility
StdSens assay. of quantitation across the quantitative
range
Before running any of the tests:
• Watch the Experion Training Video: Chip Loading (supplied with this kit and available
at www.bio-rad.com/experion/)
• Clean the electrodes as detailed in Appendix B. When working with RNA, pay special
attention to cleaning the Experion system to ensure the electrodes remain free from
RNases. This is particularly true if one system serves many users within a laboratory
Note: If this is the first time the Experion electrophoresis station is being used, refer to
Appendix C for instructions on how to prepare the system for use.
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