Bio-Rad Experion Protein Analysis Kits - Manual (Page 7)

Kit Components and Storage Conditions
Experion RNA StdSens Starter Kit Components and Storage Conditions
Component Storage Conditions
Experion RNA StdSens chips, 3 Ambient
Reagents for analysis
RNA gel, 1 vial, 1,250 µl 4°C
RNA loading buffer, 1 vial, 900 µl 4°C
RNA stain, 1 vial, 20 µl 4°C
Spin filters, 1 4°C
RNA ladder, 1 vial, 20 µl –70°C
Mouse liver total RNA sample (500 ng/µl), 20 µl –70°C
DEPC-treated water, 50 ml Ambient
Experion electrode cleaner, 25 ml Ambient
Experion cleaning chips, 2 Ambient
Foam swabs, 25 Ambient
Narrow-bore 20 µl pipet tips, RNase-free, 96 (1 rack) Ambient
0.65 ml microcentrifuge tubes, RNase-free, 50 Ambient
Experion Training Video: Chip Loading (DVD) Ambient
Instructions Ambient
Additional Materials and Equipment Required
• Experion automated electrophoresis station
• Experion priming station
• Experion vortex station
• Microcentrifuge (1,000–10,000 x g)
• Aluminum foil
• Heating block or water bath set at 70°C
• Benchtop vortexer
• Extra spin filter for additional filtration steps (optional, catalog #700-7254)
• Ice
The Experion RNA StdSens Starter Kit Tests
The Experion RNA StdSens starter kit includes all the reagents, consumables, and
instructions required to perform two tests that demonstrate the performance of the
Experion system for RNA analysis. Each test uses various dilutions and preparations of a
mouse liver total RNA sample, and each test follows the standard Experion RNA StdSens
assay workflow, which is summarized below.
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